| # | Pos. | Player | Date of Birth (Age) | Caps | Goals | Club |
|---|
| 1 | GK | Petr Čech | 20 May 1982 (age 30) | 89 | 0 |  Chelsea |
| 16 | GK | Jan Laštůvka | 7 July 1982 (age 29) | 1 | 0 |  Dnipro Dnipropetrovsk |
| 23 | GK | Jaroslav Drobný | 18 October 1979 (age 32) | 6 | 0 |  Hamburg |
|
| 2 | DF | Theodor Gebre Selassie | 24 December 1986 (age 25) | 9 | 0 |  Slovan Liberec |
| 3 | DF | Michal Kadlec | 13 December 1984 (age 27) | 33 | 7 | Bayer Leverkusen |
| 4 | DF | Marek Suchý | 29 March 1988 (age 24) | 4 | 0 |  Spartak Moscow |
| 5 | DF | Roman Hubník | 6 June 1984 (age 27) | 21 | 2 | Hertha Berlin |
| 6 | DF | Tomáš Sivok | 15 September 1983 (age 28) | 25 | 3 |  Beşiktaş |
| 20 | DF | David Limberský | 6 October 1983 (age 28) | 8 | 0 | Viktoria Plzeň |
|
| 10 | MF | Tomáš Rosický | 4 October 1980 (age 31) | 85 | 20 | Arsenal |
| 11 | MF | Milan Petržela | 19 June 1983 (age 28) | 10 | 0 | Viktoria Plzeň |
| 12 | MF | František Rajtoral | 12 March 1986 (age 26) | 2 | 0 | Viktoria Plzeň |
| 13 | MF | Jaroslav Plašil | 5 January 1982 (age 30) | 70 | 6 |  Bordeaux |
| 14 | MF | Václav Pilař | 13 October 1988 (age 23) | 8 | 1 | Wolfsburg |
| 17 | MF | Tomáš Hübschman | 4 September 1981 (age 30) | 42 | 0 | Shakhtar Donetsk |
| 18 | MF | Daniel Kolář | 27 October 1985 (age 26) | 10 | 1 | Viktoria Plzeň |
| 19 | MF | Petr Jiráček | 2 March 1986 (age 26) | 7 | 1 | Wolfsburg |
| 22 | MF | Vladimír Darida | 8 August 1990 (age 21) | 1 | 0 | Viktoria Plzeň |
|
| 7 | FW | Tomáš Necid | 13 August 1989 (age 22) | 26 | 7 | CSKA Moscow |
| 9 | MF | Jan Rezek | 5 May 1982 (age 30) | 12 | 3 |  Anorthosis Famagusta |
| 15 | FW | Milan Baroš | 28 October 1981 (age 30) | 88 | 41 | Galatasaray |
| 20 | FW | Tomáš Pekhart | 26 May 1989 (age 23) | 9 | 0 | Nürnberg |
| 21 | FW | David Lafata | 18 September 1981 (age 30) | 17 | 3 | Baumit Jablonec |
